human primary brain vascular pericyte cells Search Results


human brain vascular pericytes (pcs, sciencell)  (ScienCell)
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ScienCell human brain vascular pericytes (pcs, sciencell)
( a ) Experimental setup for human <t>pericyte</t> cell culture with reverse-transcriptase quantitative PCR (RT-qPCR) and extracellular TIMP3 protein ELISA readouts at four-timepoints. ( b ) TIMP3 protein secretion per cell per hour does not significantly change throughout culture time, even though the total protein measured by BCA does change. ( c ) qPCR experiment design with proximal and distal qPCR primers to distinguish long and short 3’ UTR isoforms. The proximal qPCR primer can detect both long and short isoforms while the distal primer can only amplify the long 3’ UTR. ( d ) The ratio of distal to proximal primer-template abundances significantly decreases throughout culture time, implying increased usage of the short TIMP3 3’ UTR compared to the long isoform. ( e ) TIMP3 3’ UTR abundance, normalized by 18 s housekeeper abundance, fluctuates from halving to doubling between culture timepoints for both distal and proximal primers.
Human Brain Vascular Pericytes (Pcs, Sciencell), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human brain vascular pericytes (pcs, sciencell) - by Bioz Stars, 2026-10
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( a ) Experimental setup for human pericyte cell culture with reverse-transcriptase quantitative PCR (RT-qPCR) and extracellular TIMP3 protein ELISA readouts at four-timepoints. ( b ) TIMP3 protein secretion per cell per hour does not significantly change throughout culture time, even though the total protein measured by BCA does change. ( c ) qPCR experiment design with proximal and distal qPCR primers to distinguish long and short 3’ UTR isoforms. The proximal qPCR primer can detect both long and short isoforms while the distal primer can only amplify the long 3’ UTR. ( d ) The ratio of distal to proximal primer-template abundances significantly decreases throughout culture time, implying increased usage of the short TIMP3 3’ UTR compared to the long isoform. ( e ) TIMP3 3’ UTR abundance, normalized by 18 s housekeeper abundance, fluctuates from halving to doubling between culture timepoints for both distal and proximal primers.

Journal: eLife

Article Title: Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation

doi: 10.7554/eLife.87517

Figure Lengend Snippet: ( a ) Experimental setup for human pericyte cell culture with reverse-transcriptase quantitative PCR (RT-qPCR) and extracellular TIMP3 protein ELISA readouts at four-timepoints. ( b ) TIMP3 protein secretion per cell per hour does not significantly change throughout culture time, even though the total protein measured by BCA does change. ( c ) qPCR experiment design with proximal and distal qPCR primers to distinguish long and short 3’ UTR isoforms. The proximal qPCR primer can detect both long and short isoforms while the distal primer can only amplify the long 3’ UTR. ( d ) The ratio of distal to proximal primer-template abundances significantly decreases throughout culture time, implying increased usage of the short TIMP3 3’ UTR compared to the long isoform. ( e ) TIMP3 3’ UTR abundance, normalized by 18 s housekeeper abundance, fluctuates from halving to doubling between culture timepoints for both distal and proximal primers.

Article Snippet: Human brain vascular pericytes (PCs, Sciencell) were cultured up to passage 5 in low-glucose DMEM (Gibco) supplemented with 10% FBS.

Techniques: Cell Culture, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: eLife

Article Title: Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation

doi: 10.7554/eLife.87517

Figure Lengend Snippet:

Article Snippet: Human brain vascular pericytes (PCs, Sciencell) were cultured up to passage 5 in low-glucose DMEM (Gibco) supplemented with 10% FBS.

Techniques: Software, Sequencing, Enzyme-linked Immunosorbent Assay